Anthelmintic and Antioxidant Activities, Phytochemical Profile and Microscopic Features of Senna alata Collected in the Democratic Republic of Congo

Aim: The objective of this study was to determine the phytochemical profile of Senna alata LINN using chemical screening in solution and thin-layer chromatography, and to assess the antioxidant and anthelmintic activities of the plant抯 aqueous extracts. Methodology: All the analyses performed in this study were, respectively, done as described by the standard protocols. These were: the microscopic examination of the plant powders performed using a light microscope, the search for secondary metabolites carried out by chemical screening in solution and by thin-layer chromatography, the determination of the secondary metabolites, and the antioxidant activity carried out by UV-visible spectroscopy and the anthelmintic activity performed by dilution in decreasing order of concentration. Results: Micrographic analysis of the powder of Senna alata revealed the histological elements rich in unicellular covering hairs with a punctate surface and in fragments of palisade parenchyma, with elongated cells. The presence of polyphenols (flavonoids, anthocyanins, tannins, leuco anthocyanins, free quinones), steroids, terpenoids, and iridoids was detected by phytochemical screening in solution and confirmed by thin-layer chromatography. The determination of total phenolic compounds, flavonoids, total tannins, and anthocyanins showed that Senna alata contains 254.64 mg EQ/g, 12.3%, 9.5%, and 6.5%, respectively, of these metabolites. The aqueous extract of the leaves of Senna alata showed a good anthelmintic activity after 41 minutes of exposure to 5.00 mg/mL of the extract and the antioxidant activity was reported, of which the value of IC50 ( g/mL) of the extract for the DPPH tests is 91.42 15.56. Conclusion: Histological elements rich in unicellular covering hairs with a punctate surface and in fragments of palisade parenchyma, with elongated cells were revealed in the micrographic analysis of Senna alata. The plant抯 leaf methanol extract showed good antioxidant activity, while the anthelmintic activity was demonstrated in its aqueous extract.

Anthelmintic activity of seaweed extract of Padina boergesenii and Padina tetrastromatica acute subacute level in naturally infected sheep

Background: Seaweeds have showed a broad range of biological activities such as antiviral, cardiovascular, autoimmune diseases, antineoplastic antibacterial, anti-inflammatory, and antifungal activities. Aims and Objectives: This study was done to assess capability of Padina boergesenii and Padina tetrastromatica extract for anthelmintic activity in sheep agonized with clinical parasitic gastroenteritis in different concentrations and compared it against the standard drug albendazole using natural acute/subacute parasitic gastroenteritis due primarily to mixed nematode species. Materials and Methods: The anthelmintic activity of ethanolic extract of seaweed of P. boergesenii was studied in sheep with natural acute/subacute parasitic gastroenteritis due primarily to mixed nematode species. Graded doses of the extract (100, 200, and 300 mg/kg, p.o for 5 consecutive days) significantly reduced fecal egg counts in infected animals. The percentage reduction (96.1%) by 300 mg/kg of the extract was comparable to that of 5 mg/kg of albendazole (94.3%). Results: The administration of the extract resulted in improved hemoglobin and leukocytosis values in worm infected sheep. Conclusion: The current study evidence that the anthelmintic activity of the ethanolic extract of seaweed of P. boergesenii and P. tetrastromatica has promising anthelmintic activity against strongyle, Strongyloides papillosus, and Taenia ovis. However, the anthelmintic activity of P. boergesenii was superior to P. tetrastromatica.

Synthesis and Pharmacological Activity of Some Pyrazolone Derivatives

A sequence of novel pyrazolone derivative was produced by the reaction of 2, 4-dinitrophenyl hydrazine (0.1 mol) and ethyl acetoacetate with benzaldehydes followed by hydrazine hydrate and finally treated with secondary amine and formaldehyde and the synthesized compounds were characterized by their physical properties (M.P and TLC) and UV, IR, 1HNMR, Mass spectroscopic studies respectively. The entire synthesized complex was tested for their anthelmintic, antimicrobialand haemostatic activity against gram-positive and gram-negative strains of bacteria and Eudrilus eugeniaand human venous blood. The antimicrobial activity of synthesized pyrazolone derivatives was assessed by agar cup method. All the synthesized complexes were screened for the antimicrobial, athelminticand haemostatic activity against some gram (+ve), Gram(-ve) organisms, Eudrilus eugeniaand human venous blood. The complexes exhibited reasonable to upright activity when compared with the standard one.

Synthesis of Some Novel Tetra Aryl Imidazoles Compounds: Evaluation of In-Vitro Cytotoxicity and Anthelmintic Activity

The imidazole derivatives have potent therapeutic activity against cytotoxicity and parasites. The present study was planned to synthesize novel tetra aryl imidazoles compounds and evaluated for in vitro cytotoxicity and anthelmintic activity. Firstly, 2-amine-4-chloro pyridine was condensed with substituted benzaldehyde to give corresponding Schiff’s base. These Schiff’s bases further on treatment with ammonium acetic acid derivation and isatin yielded comparing novel tetra aryl imidazoles. The synthesized compounds were examined for in-vitro cytotoxicity and anthelmintic activity. The discoveries showed that all the synthesized novel substituted imidazoles have moderate to great anthelmintic action. They additionally had critical in-vitro cytotoxicity against HEp2 cell lines (Human larynx malignancy cell line) against standard utilizing 5-fluorouracil. The compounds 1b, 2b, 4b, 6b, and 8b had higher anthelmintic action contrasted with standard mebendazole. The synthesized compounds 1b, 2b and 8b had noteworthy in-vitro cytotoxicity against HEp2 cell lines.

Comparative Phytochemical Screening and In vitro Evaluation of Biological Activities between Aqueous and Ethanolic Extract of Momordica charantia L. Fruits.

Aims: The study was conducted to compare the presence of different phytochemicals and biological activities like cytotoxicity, anthelmintic activity, antioxidant and free radical scavenging activities between aqueous and ethanolic extract of Momordica charantia L. Fruits. Methodology: The cytotoxic assay was undertaken using brine shrimp lethality test (BSLT) while the anthelmintic activity was carried out with the determination of time of paralysis and death of earthworm (Pheritima posthuma) at five different concentrations. Antioxidant and free radical scavenging activities were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and determining the total phenolic contents. Results: Study revealed that several phytochemicals were found common for both of the extracts, though proteins and amino acids were only found in ethanolic extract. Both the extracts showed mild cytotoxic activity where the ethanolic extract showed better potency (LC50=24.245 μg/ml) than aqueous extract (LC50=24.515 μg/ml). In case of anthelmintic activity, ethanolic extract was also found significantly potent than aqueous extract at five different concentrations. IC50 values for the total antioxidant activity were 304.41±0.903 μg/ml and 479.05±1.393 μg/ml for ethanolic and aqueous extract, respectively. Ethanolic extract contained a significantly higher concentration of total phenols (71.08 ± 0.380 mg of GAE/g of extract) in comparison to aqueous extract (57.33±0.520 mg of GAE/g of extract). Conclusion: Therefore, in all aspects of the study, ethanolic extract was found more potent than aqueous extract. It can be concluded that M. charantia fruits are abundant of various phytochemicals and possess versatile biological activities.

In-vitro Evaluation of Cytotoxic and Anthelmintic Activity of Luffa acutangula, Luffa aegyptiaca and Momordica cochinchinensis.

Aims: The study was designed to investigate cytotoxic and anthelmintic activity of aerial parts of Luffa acutangula (L.) Roxb. (Family: Cucurbitaceae, locally known as ‘Jhinga’), Luffa aegyptiaca Mill. (Family: Cucurbitaceae, locally known as ‘Dhundul’) and Momordica cochinchinensis (Lour.) Spreng. (Family: Cucurbitaceae, locally known as ‘Kakrol’) extracted with various solvents (petroleum ether & methanol). Study Design: Determination of cytotoxic and anthelmintic activity of aerial parts of three (Cucurbitaceae family) Bangladeshi plants. Place and Duration of Study: Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342. Performed between November 2011- September 2012. Methodology: The cytotoxic activity was evaluated by Brine Shrimp lethality bioassay and anthelmintic activity by in-vitro test using earth worm Pheretima posthuma (Annelida) as test animals. Results: In Brine Shrimp lethality bioassay, methanol extract of M. cochinchinensis and L. aegyptiaca were found to be highly toxic to Brine Shrimp nauplii, having LC50 of 1.91±0.79 μg/ml and 3.97±0.61 μg/ml respectively. The three methanol extracts of aerial part of L. acutangula, L. aegyptiaca and M. cochinchinensis showed moderate anthelmintic activity. 50mg/ml concentration of methanol extract of M. cochinchinensis showed maximum activity showing death in test animals at 43±1.3 min which is comparable to the standard (Piperazine Citrate, 10 mg/ml) which killed the test animal at 38 ± 0.63 min. Conclusion: Further studies are suggested to be undertaken to understand the underlying mechanism of the observed cytotoxic and anthelmintic activity of these three Bangladeshi (Cucurbitaceae family) plants.

Anthelmintic activity of Cassia occidentalis L. methanolic leaf extract on Ascaridia galli and Heterakis gallinarum and its acute toxicity.

Background: Cassia occidentalis is traditionally used to treat helminth infestations in poultry. We investigated the in-vitro anthelmintic activity of the methanolic leaf extract of this plant against Heterakis gallinarum and Ascaridia galli worms and its acute toxicity. Methods: Leaves of the plant were air dried, ground into powder and extracted with 70% methanol, filtered and dried at 50℃ into a dark green semi-solid mass. The worms were isolated from fresh intestines of local chicken and pheasants or turkeys freshly slaughtered in Kampala markets. They were placed in conical flasks containing Goodwin’s solution, kept in a water bath set at 39°C and exposed to 8, 12, 16, 20 and 24mg/ml extract concentrations. Percent worm mortality was determined for each concentration at 12h intervals. piperazine and ivermectin were used as positive controls. For acute toxicity evaluation, Swiss mice were divided into 5 groups (n=6) and administered per os with extract doses of 5,000, 10,000, 15,000, 20,000 and 25,000mg/Kg. The EC50 was determined by a plot of mortality probits against log extract concentration. Results There was a concentration-dependent relationship with worm mortality. The mean worm mortality for A. galli was significantly higher for the extract than for piperazine at 16 and 20mg/ml (p<0.05) and 24 mg/ml concentrations (p<0.01). Activity against H. gallinarum was not significantly different from that of ivermectin. The EC50 of the extract was 11.78mg/ml for A. galli and 17.78mg/ml for H. gallinarum. The extract is safe according to OECD acute toxicity guidelines since no mortality and toxicity signs were observed in mice even at 25,000mg/kg. Conclusions: The study demonstrated the anthelmintic activity of C. occidentalis and this could explain its use in traditional medicine as a remedy against helminth infections over the generations in many parts of Uganda.

Anthelmintic, antimicrobial, antioxidant and cytotoxic activity of Caltha palustris var. alba Kashmir, India / 中国天然药物

The methanolic extract obtained from the root portion of Caltha palustris var. alba was evaluated for its anthelmintic efficacy against gastrointestinal nematodes of sheep under both in vitro and in vivo conditions using worm motility inhibition (WMI) assay and fecal egg count reduction (FECR) assay, respectively. The extract was subjected to antimicrobial activity using agar-well diffusion method against different bacterial strains. In addition the extract was evaluated for cytotoxic and antioxidant activity against cultured THP-1(Leukemia), A-549 (Lung), HCT-15 (Colon), Cervix (HeLa) and PC-3(Prostrate) cell lines by SRB and DPPH radical scavenging assays. The extract used resulted in mean %WMI of 94.44%, as observed when the worms were put in lukewarm buffer for 30 min after exposure to different treatments. The mean mortality index of the sample was 0.95. The lethal concentration (LC50) was 0.11 mg·mL(-1). Cell lines were exposed to concentration of 100 μg·mL(-1) of extract for 48 h, which reduced the viability of these cell lines. The same plant extract also showed 55.58% DPPH radical scavenging activity.

Anthelmintic activity of methanolic extract of rhizomes of picrorrhiza kurroa royal ex. Benth.

The objective of this study is to evaluate and compare the Anthelmintic activity of methanolic extract of Picrorrhiza kurroa Royle ex. Benth (Scrophhulariaceae). Picrorrhiza kurroaia a small perennial herb growing in the hilly parts of the North-western Himalayan region in India and Nepal. Earth worms were used for Anthelmintic activity. Piperazine citrate was used as standard drug. Time required for paralysis and death of the earth worms were noted for each sample.

In–Vitro Anthelmintic Activity of Pet – Ether Extract of Portulaca Oleracea (LINN.) Against Pheritima Posthuma.

Present study was carried out to investigate the anthelmintic potential of petroleum-ether extract of Portulaca oleracea against Pheretima posthuma as a species of earthworm. Various concentrations (25‐100 mg/ml) of petroleum-ether extract were evaluated for anthelmintic activity by recording the time required for paralysis and death of worms. Albendazole was used as standard. Result indicates that petroleum-ether extract significantly (p<0.01) exhibited paralysis at lower doses of 50, 75 and 100 mg/ml and causes death of worms at doses of concentrations 75 and 100 mg/ml when compared with standard. The present study indicates that the petroleum-ether extract of Portulaca oleracea has a potential anthelmintic activity can be used as anthelmintic drug.

Atividade anti-helmíntica de plantas nativas do continente americano: uma revisão / Anthelmintic activity of native American plants: a review

O presente trabalho faz uma revisão de plantas nativas do continente americano com atividade anti-helmíntica com a finalidade de subsidiar pesquisas e o desenvolvimento de novos medicamentos na área de produtos naturais. Na literatura, foram citadas 22 famílias de plantas, tais como: Moraceae, Asteraceae e Cucurbitaceae, e estudadas cerca de 35 espécies, como a Ficus sp, Cucurbita máxima, e Ruta graveolens, usadas por sua alegada atividade anti-helmíntica. Destas espécies, 26 foram ativas contra alguns helmintos: Strongyloides stercolaris, Ancylostomidae, Trichostrongylus sp, Capillaria sp, Ascaris lumbricoides, Taenia saginata, Haemonchus contortus, e Hymenolepsis diminuta. Foi verificado maior número de estudos com parasitas de animais, possivelmente devido ao desenvolvimento de resistência dos helmintos aos tratamentos convencionais disponíveis.

This paper is a review of native American plants showing anthelmintic activity in order to give support to research and development of new drugs in the field of natural products. In the literature, 22 plant families were cited, such as: Moraceae, Asteraceae and Cucurbitaceae, and around 35 species were studied, including Ficus sp, Cucurbita maxima and Ruta graveolens, used for their putative anthelmintic activity. From these species, 26 were active against some helminthes: Strongyloides stercolaris, Ancylostomidae, Trichostrongylus sp, Capillaria sp, Ascaris lumbricoides, Taenia saginata, Haemonchus contortus and Hymenolepsis diminuta. Data indicated a larger number of studies with animal parasites, probably due to the resistance of these helminthes to the conventional treatments available.

Anthelmintic and diuretic activity of bark extracts of Sterculia villosa.

The study was evaluated for diuretic and anthelmintic activity of the ethanolic extract of the barks of Sterculia villosa Roxb, (Sterculiaceae). The diuretic assay was done on both healthy wistar rats and rabbits. The dose used for the diuretic assay was 100, 200 & 400mg/kg of the extract. Compared to the control and standard drug furosemide (20mg/kg), the result of diuretic study showed dose dependent activity of the extracts. The result also indicated higher excretion of Na+, K+ & Cl- in urine. Pheretima posthuma, adult earthworms were used for anthelmintic activity and the results of anthelmintic assessment at the dose of 50, 100 & 200mg/ml showed significant activity compared with control and standard drug albendazole (10mg/ml).

Plantas taniníferas e o controle de nematóides gastrintestinais de pequenos ruminantes / Tanniferous plants and control of gastrointestinal nematodes of small ruminants

Os nematóides gastrintestinais são responsáveis por acentuada redução na produtividade de ovinos e caprinos. Na tentativa de controlar esses parasitos, são utilizados diversos anti-helmínticos sintéticos, mas o desenvolvimento da resistência tem tornado esse controle pouco eficaz. Na busca por alternativas de controle dos nematóides gastrintestinais, a utilização de plantas taniníferas tem despertado interesse de pesquisadores em várias regiões do mundo. Essa revisão visa a abordar os diversos aspectos relacionados ao fornecimento dessas plantas a ovinos e caprinos, bem como os principais resultados obtidos em pesquisas utilizando taninos para o controle de nematóides de pequenos ruminantes.

The gastrointestinal nematodes are responsible for a marked reduction in the productivity of sheep and goats. In an attempt to control these parasites, different synthetic anthelmintics are used, but development of resistance has become this control little effective. In the search for alternatives to control these nematodes, the use of tanniferous plants has attracted attention from researchers in different regions of the world. This review aims to address the various aspects related to the administration of these plants to sheep and goats, and the main results obtained from studies using tannins to control nematodes in small ruminants.

Investigation of in-vitro anthelmintic activity of Bauhinia racemosa linn.

Helminth infections are the most common health problems in India, in developing countries they pose a large treat to public. These infections can affect most population in endemic areas with major economic and social consequences. The plant Bauhinia Racemosa Linn. is a species of flowering plant belongs to Fabaceae family. The different parts of plant being traditionally used in catarrh, infection of children, boil, glandular and swelling. The present study was undertaken to evaluate anthelmintic activity of different extracts of whole plant of Bauhinia Racemosa Linn. The different successive extracts namely petroleum ether, ethanol and aqueous using an adult Indian earthworms, Pheretima posthuma as a test worm. Three concentrations (50, 75 and 100 mg/ml) of each extracts were studied in the bioassay which involved the determination of time of paralysis and time of death of the worm. Albenzadole in same concentration as that of extract was included as standard reference and normal saline water as control. The results of present study indicate that the crude ethanolic extract significantly demonstrated paralysis and also caused death of worm in dose dependent manner, while aqueous and petroleum extracts show weak anthelmintic effect. Further studies are in process to isolate the active principles responsible for the activity.

Anthelmintic activity study of saraca indica leaves extracts.

To know the anthelmintic activity of the leaves of Saraca indica, present study was conducted. For this work, we extracted the leaves with methanol and ethanol by following the maceration and the soxhlet methods of extraction. In case of the ethanolic extract obtained from the maceration method, it was found that its anthelmintic activity was more than the methanolic extract. On the other hand, the methanolic extract, obtained from the soxhlet method of extraction, indicated that it had better anthelmintic activity than the ethanolic extract. Overall the anthelmintic activity revealed the concentration-dependent nature of the extracts. In cases of both the methods of extraction, it was observed that both the extracts had more potent anthelmintic activity than Piperazine citrate (positive control).

Efeito in vitro do extrato etanólico das folhas do melão-de-São-Caetano (Momordica charantia L. ) sobre ovos e larvas de nematóides gastrintestinais de caprinos / In vitro effect of the ethanolic extract of "melão de São Caetano" (Momordica charantia L. ) leaves on the eggs and larvae of gastrointestinal nematodes from goats

O experimento in vitro foi realizado para avaliar a ação do extrato etanólico das folhas do melão-de-São-Caetano (Momordica charantia L.) sobre o desenvolvimento de ovos e motilidade de larvas de nematóides gastrintestinais de caprinos. As larvas foram obtidas de coproculturas e a recuperação de ovos foi feita pela técnica dos quatro tamises, a partir de fezes de caprinos naturalmente infectados da mesorregião do Sertão Paraibano. O extrato foi utilizado nas diluições de 50; 25; 12,5; 6,25 e 3,12 por cento para ambos os testes e como controle positivo e para controle negativo, utilizou-se água destilada estéril. As placas foram examinadas ao microscópio óptico para contagem dos ovos em desenvolvimento e larvas móveis e imóveis, após 24, 48 e 72 horas de incubação. As concentrações do extrato etanólico de M. charantia e os tratamentos controle negativo e positivo diferiram quanto ao número de ovos inviáveis. No teste de motilidade larval as concentrações acima de 12 por cento apresentaram médias significativas quanto ao número de larvas inviáveis. Nas condições ensaiadas a M. charantia apresentou atividade ovicida e larvicida.

The experiment in vitro was performed to evaluate the action of the ethanolic extract of "melão de São Caetano" (Momordica charantia L.) leaves on the development of eggs and motility of larvae of gastrointestinal nematodes from goats. The nematode larvae were obtained from coproculture and the recovery of eggs was done in sieves, from feces of naturally infected goats from the Mesoregion of Paraíba State. The extract was used at the dilutions of 50, 25, 12.5, 6.25 and 3.12 percent for both tests and as positive control; for negative control, sterile distilled water was used. The plates were examined under optical microscope to count the eggs in development and mobile larvae after 24, 48 and 72 h of incubation. The concentrations of M. charantia ethanolic extract and the negative and positive controls differed as to the number of eggs that were not viable. In the larval motility test, concentrations higher than 12 percent had significant means as to the number of larvae that were not viable. Under the tested conditions, M. charantia showed larvicidal and ovicidal activity.

Atividade anti-helmíntica dos extratos aquoso e etanólico do fruto da Morinda citrifolia sobre Ascaridia galli / Anthelmintic activity of aqueous and ethanolic extracts of Morinda citrifolia fruit on Ascaridia galli

A atividade anti-helmíntica dos extratos aquoso e etanólico do fruto da Morinda citrifolia (noni) foi avaliada em aves poedeiras naturalmente infectadas por Ascaridia galli. A atividade anti-helmíntica in vitro foi determinada em parasitos adultos. O extrato aquoso e etanólico foram testados nas seguintes concentrações: 1,69; 3,37; 6,74; 13,48 e 26,96 mg.mL-1 e 4,17; 8,34; 16,68; 33,36 e 66,72 mg.mL-1, respectivamente. A atividade anti-helmíntica in vivo foi determinada administrando-se, durante três dias consecutivos, o extrato aquoso (50,1 mg.mL-1) e etanólico (24,6 mg.mL-1), sendo 10 mL.kg-1. Posteriormente, as aves foram sacrificadas e necropsiadas para contagem dos helmintos remanescentes. Os dados obtidos foram analisados estatisticamente, utilizando-se o teste de Student-Newman-Keuls. Nas concentrações 13,48 e 26,96 mg.mL-1, o extrato aquoso apresentou taxa de mortalidade de 46,67 e 50 por cento, respectivamente, sendo estatisticamente diferente do controle negativo (P < 0,05). O extrato etanólico apresentou diferença significativa do controle negativo (diluente) (P < 0,05) para as concentrações 33,36 e 66,72 mg.mL-1, expressando taxa de mortalidade de 66,67 e 76,67 por cento, respectivamente. No teste in vivo, o extrato aquoso do fruto do noni apresentou percentual de eliminação de 27,08 por cento, diferindo estatisticamente do grupo controle. Não houve diferença significativa entre os tratamentos com extrato etanólico e controle (P > 0,05). Conclui-se que a atividade anti-helmíntica do fruto do noni, no teste in vitro, apresentou resultados satisfatórios, havendo necessidade de estudos com maiores concentrações no teste in vivo.

The anthelmintic activity of aqueous and ethanolic extracts of Morinda citrifolia fruit (noni) was evaluated in chicken naturally infected by Ascaridia galli. The anthelmintic activity in vitro was determined in adult parasites. The aqueous and ethanolic extracts were used in the following concentrations: 1.69; 3.37; 6.74; 13.48 e 26.96 mg.mL-1 and 4.17; 8.34; 16.68; 33.36 and 66.72 mg.mL-1, respectively. The anthelmintic activity in vivo was determined by the administration of 10 mL.kg-1 of the aqueous (50.1 mg.mL-1) and ethanolic (24.6 mg.mL-1) extracts during three consecutive days. Later the chickens were euthanized and necropsy was performed in order to count the remaining helminths. The data were analyzed by the Student-Newman-Keuls test. In the concentrations of 13.48 and 26.96 mg.mL-1, the aqueous extract demonstrated mortality of 46.67 and 50 percent, respectively, there was a significative difference from the negative control (P < 0.05). The ethanolic extract presented statistical difference from the negative control (diluent) (P < 0.05) for the concentrations of 33.36 and 66.72 mg.mL-1, expressed by a mortality rate of 66.67 and 76.67 percent, respectively. In the in vivo test, the aqueous extract of noni fruit showed 27.08 percent of elimination, deferring statistically from the control group. There was no statistical difference between the ethanolic extract treatments and the control (P > 0.05). It follows that the anthelmintic activity of noni fruit test showed satisfactory results in vitro, there is a need for studies in higher concentrations in the in vivo test.

Atividade anti-helmíntica dos flavonóides isolados das raízes de Andira anthelmia (Leguminosae) / Anthelmintic activity of flavonoids isolated from roots of Andira anthelmia (Leguminosae)

As cascas e as raízes de A. anthelmia têm sido utilizadas tradicionalmente como vermífugas. Um estudo biomonitorado do extrato metanólico das raízes de A. anthelmia conduziu ao isolamento das isoflavonas biochanina A e genisteína da fração acetato de etila; biochanina A 7-O-β-D-glicopiranosídeo, biochanina A 7-O-α-L-rhamnopiranosil-(1→6)-β-D-glicopiranosídeo e biochanina A 7-O-β-D-apiofuranosil-(1→5)-β-D-apiofuranosil-(1→6)-β-D-glicopiranosídeo da fração n-butanólica e catequina da fração metanólica. Suas estruturas foram elucidadas com base em dados espectrométricos. A atividade anti-helmíntica foi investigada em camundongos naturalmente infectados por Aspiculuris tetraptera. Os compostos isolados aplicados nos animais por via intragástrica na dose de 2,0 mg.kg-1 durante três dias consecutivos revelaram percentuais significativos na eliminação de A. tetraptera, quando comparados com o lote controle.

Bioactivity-guided fractions of the methanol extract from the roots of Andira anthelmia led the isolation of the isoflavones biochanin A and genistein from the ethyl acetate fraction; biochanin A 7-O-β-D-glucopyranoside, biochanin A 7-O-α-L-rhamnopyranosyl-(1→6)β-D-glucopyranoside and biochanin A 7-O-β-D-apiofuranosyl-(1→5)-β-D-apiofuranosyl-(1→6)β-D-glucopyranoside from the n-butanol fraction and catechin from the methanol one. Their structures were elucidated on the basis of spectroscopy data. The anthelmintic activity was investigated in mice naturally infected by Aspiculuris tetraptera. The compounds administered in the animals by intragastric route in doses of 2.0 mg.kg-1, were effective in the removal of the total number of the A. tetraptera when compared with the control group.